Infectious Middle East Respiratory Syndrome Coronavirus Excretion and Serotype Variability Based on Live Virus Isolates from Patients in Saudi Arabia.
Identifieur interne : 001746 ( Main/Exploration ); précédent : 001745; suivant : 001747Infectious Middle East Respiratory Syndrome Coronavirus Excretion and Serotype Variability Based on Live Virus Isolates from Patients in Saudi Arabia.
Auteurs : Doreen Muth [Allemagne] ; Victor M. Corman [Allemagne] ; Benjamin Meyer [Allemagne] ; Abdullah Assiri ; Malak Al-Masri ; Mohamed Farah ; Katja Steinhagen [Allemagne] ; Erik Lattwein [Allemagne] ; Jaffar A. Al-Tawfiq [États-Unis] ; Ali Albarrak ; Marcel A. Müller [Allemagne] ; Christian Drosten [Allemagne] ; Ziad A. Memish [Allemagne]Source :
- Journal of clinical microbiology [ 1098-660X ] ; 2015.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Animaux, Arabie saoudite (épidémiologie), Cellules Caco-2, Cellules Vero, Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Culture virale, Femelle, Humains, Infections à coronavirus (virologie), Infections à coronavirus (épidémiologie), Jeune adulte, Mâle, Sujet âgé, Sujet âgé de 80 ans ou plus, Sérogroupe.
- MESH :
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Infections à coronavirus.
- épidémiologie : Arabie saoudite, Infections à coronavirus.
- Adulte, Adulte d'âge moyen, Animaux, Cellules Caco-2, Cellules Vero, Coronavirus du syndrome respiratoire du Moyen-Orient, Culture virale, Femelle, Humains, Jeune adulte, Mâle, Sujet âgé, Sujet âgé de 80 ans ou plus, Sérogroupe.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Adult, Aged, Aged, 80 and over, Animals, Caco-2 Cells, Chlorocebus aethiops, Coronavirus Infections (epidemiology), Coronavirus Infections (virology), Female, Humans, Male, Middle Aged, Middle East Respiratory Syndrome Coronavirus (classification), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Saudi Arabia (epidemiology), Serogroup, Vero Cells, Virus Cultivation, Young Adult.
- MESH :
- geographic , epidemiology : Saudi Arabia.
- classification : Middle East Respiratory Syndrome Coronavirus.
- epidemiology : Coronavirus Infections.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- virology : Coronavirus Infections.
- Adult, Aged, Aged, 80 and over, Animals, Caco-2 Cells, Chlorocebus aethiops, Female, Humans, Male, Middle Aged, Serogroup, Vero Cells, Virus Cultivation, Young Adult.
Abstract
The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) has infected at least 1,082 people, including 439 fatalities. So far, no empirical virus isolation study has been done to elucidate infectious virus secretion or serotype variability. Here, we used 51 respiratory samples from 32 patients with confirmed MERS-CoV infection for virus isolation in Vero B4 and Caco-2 cells. We found Caco-2 cells to significantly enhance isolation success over routinely used Vero cells. Isolation success correlated with viral RNA concentration and time after diagnosis as well as with the amount of IgA antibodies secreted in respiratory samples used for isolation. Results from plaque reduction neutralization assays using a representative range of serum samples and virus isolates suggested that all circulating human MERS-CoV strains represent one single serotype. The choice of prototype strain is not likely to influence the success of candidate MERS-CoV vaccines. However, vaccine formulations should be evaluated for their potential to induce IgA.
DOI: 10.1128/JCM.01368-15
PubMed: 26157150
Affiliations:
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Corman</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Virology, University of Bonn Medical Centre, Bonn, Germany German Centre for Infection Research (DZIF), Bonn-Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology, University of Bonn Medical Centre, Bonn, Germany German Centre for Infection Research (DZIF), Bonn-Cologne</wicri:regionArea><wicri:noRegion>Bonn-Cologne</wicri:noRegion><wicri:noRegion>Bonn-Cologne</wicri:noRegion><wicri:noRegion>Bonn-Cologne</wicri:noRegion></affiliation></author><author><name sortKey="Meyer, Benjamin" sort="Meyer, Benjamin" uniqKey="Meyer B" first="Benjamin" last="Meyer">Benjamin Meyer</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology, University of Bonn Medical Centre, Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Assiri, Abdullah" sort="Assiri, Abdullah" uniqKey="Assiri A" first="Abdullah" last="Assiri">Abdullah Assiri</name><affiliation><nlm:affiliation>Ministry of Health, Riyadh, Kingdom of Saudi Arabia.</nlm:affiliation><wicri:noCountry code="subField">Kingdom of Saudi Arabia</wicri:noCountry></affiliation></author><author><name sortKey="Al Masri, Malak" sort="Al Masri, Malak" uniqKey="Al Masri M" first="Malak" last="Al-Masri">Malak Al-Masri</name><affiliation><nlm:affiliation>Ministry of Health, Riyadh, Kingdom of Saudi Arabia.</nlm:affiliation><wicri:noCountry code="subField">Kingdom of Saudi Arabia</wicri:noCountry></affiliation></author><author><name sortKey="Farah, Mohamed" sort="Farah, Mohamed" uniqKey="Farah M" first="Mohamed" last="Farah">Mohamed Farah</name><affiliation><nlm:affiliation>Central Military Laboratory and Blood Bank, Microbiology Division, Prince Sultan Military City, Riyadh, Kingdom of Saudi Arabia.</nlm:affiliation><wicri:noCountry code="subField">Kingdom of Saudi Arabia</wicri:noCountry></affiliation></author><author><name sortKey="Steinhagen, Katja" sort="Steinhagen, Katja" uniqKey="Steinhagen K" first="Katja" last="Steinhagen">Katja Steinhagen</name><affiliation wicri:level="1"><nlm:affiliation>EUROIMMUN AG, Lübeck, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>EUROIMMUN AG, Lübeck</wicri:regionArea><wicri:noRegion>Lübeck</wicri:noRegion><wicri:noRegion>Lübeck</wicri:noRegion><wicri:noRegion>Lübeck</wicri:noRegion></affiliation></author><author><name sortKey="Lattwein, Erik" sort="Lattwein, Erik" uniqKey="Lattwein E" first="Erik" last="Lattwein">Erik Lattwein</name><affiliation wicri:level="1"><nlm:affiliation>EUROIMMUN AG, Lübeck, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>EUROIMMUN AG, Lübeck</wicri:regionArea><wicri:noRegion>Lübeck</wicri:noRegion><wicri:noRegion>Lübeck</wicri:noRegion><wicri:noRegion>Lübeck</wicri:noRegion></affiliation></author><author><name sortKey="Al Tawfiq, Jaffar A" sort="Al Tawfiq, Jaffar A" uniqKey="Al Tawfiq J" first="Jaffar A" last="Al-Tawfiq">Jaffar A. Al-Tawfiq</name><affiliation wicri:level="2"><nlm:affiliation>Johns Hopkins Aramco Healthcare, Dahran, Kingdom of Saudi Arabia Indiana University School of Medicine, Indianapolis, Indiana, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Johns Hopkins Aramco Healthcare, Dahran, Kingdom of Saudi Arabia Indiana University School of Medicine, Indianapolis, Indiana</wicri:regionArea><placeName><region type="state">Indiana</region></placeName></affiliation></author><author><name sortKey="Albarrak, Ali" sort="Albarrak, Ali" uniqKey="Albarrak A" first="Ali" last="Albarrak">Ali Albarrak</name><affiliation><nlm:affiliation>Saudi Center for Disease Control, Ministry of Health, Riyadh, Kingdom of Saudi Arabia.</nlm:affiliation><wicri:noCountry code="subField">Kingdom of Saudi Arabia</wicri:noCountry></affiliation></author><author><name sortKey="Muller, Marcel A" sort="Muller, Marcel A" uniqKey="Muller M" first="Marcel A" last="Müller">Marcel A. Müller</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology, University of Bonn Medical Centre, Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Virology, University of Bonn Medical Centre, Bonn, Germany German Centre for Infection Research (DZIF), Bonn-Cologne, Germany drosten@virology-bonn.de zmemish@yahoo.com.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>Institute of Virology, University of Bonn Medical Centre, Bonn, Germany German Centre for Infection Research (DZIF), Bonn-Cologne</wicri:regionArea><wicri:noRegion>Bonn-Cologne</wicri:noRegion><wicri:noRegion>Bonn-Cologne</wicri:noRegion><wicri:noRegion>Bonn-Cologne</wicri:noRegion></affiliation></author><author><name sortKey="Memish, Ziad A" sort="Memish, Ziad A" uniqKey="Memish Z" first="Ziad A" last="Memish">Ziad A. Memish</name><affiliation wicri:level="1"><nlm:affiliation>Ministry of Health, Riyadh, Kingdom of Saudi Arabia College of Medicine, Alfaisal University, Riyadh, Kingdom of Saudi Arabia drosten@virology-bonn.de zmemish@yahoo.com.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>Ministry of Health, Riyadh, Kingdom of Saudi Arabia College of Medicine, Alfaisal University, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion><wicri:noRegion>Riyadh</wicri:noRegion><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="eISSN">1098-660X</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Animals</term><term>Caco-2 Cells</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (epidemiology)</term><term>Coronavirus Infections (virology)</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Middle East Respiratory Syndrome Coronavirus (classification)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Saudi Arabia (epidemiology)</term><term>Serogroup</term><term>Vero Cells</term><term>Virus Cultivation</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Arabie saoudite (épidémiologie)</term><term>Cellules Caco-2</term><term>Cellules Vero</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Culture virale</term><term>Femelle</term><term>Humains</term><term>Infections à coronavirus (virologie)</term><term>Infections à coronavirus (épidémiologie)</term><term>Jeune adulte</term><term>Mâle</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Sérogroupe</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Saudi Arabia</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Arabie saoudite</term><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Animals</term><term>Caco-2 Cells</term><term>Chlorocebus aethiops</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Serogroup</term><term>Vero Cells</term><term>Virus Cultivation</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Cellules Caco-2</term><term>Cellules Vero</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Culture virale</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Sérogroupe</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Arabie saoudite</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) has infected at least 1,082 people, including 439 fatalities. So far, no empirical virus isolation study has been done to elucidate infectious virus secretion or serotype variability. Here, we used 51 respiratory samples from 32 patients with confirmed MERS-CoV infection for virus isolation in Vero B4 and Caco-2 cells. We found Caco-2 cells to significantly enhance isolation success over routinely used Vero cells. Isolation success correlated with viral RNA concentration and time after diagnosis as well as with the amount of IgA antibodies secreted in respiratory samples used for isolation. Results from plaque reduction neutralization assays using a representative range of serum samples and virus isolates suggested that all circulating human MERS-CoV strains represent one single serotype. The choice of prototype strain is not likely to influence the success of candidate MERS-CoV vaccines. However, vaccine formulations should be evaluated for their potential to induce IgA. </div></front></TEI><affiliations><list><country><li>Allemagne</li><li>États-Unis</li></country><region><li>District de Cologne</li><li>Indiana</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Bonn</li></settlement></list><tree><noCountry><name sortKey="Al Masri, Malak" sort="Al Masri, Malak" uniqKey="Al Masri M" first="Malak" last="Al-Masri">Malak Al-Masri</name><name sortKey="Albarrak, Ali" sort="Albarrak, Ali" uniqKey="Albarrak A" first="Ali" last="Albarrak">Ali Albarrak</name><name sortKey="Assiri, Abdullah" sort="Assiri, Abdullah" uniqKey="Assiri A" first="Abdullah" last="Assiri">Abdullah Assiri</name><name sortKey="Farah, Mohamed" sort="Farah, Mohamed" uniqKey="Farah M" first="Mohamed" last="Farah">Mohamed Farah</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Muth, Doreen" sort="Muth, Doreen" uniqKey="Muth D" first="Doreen" last="Muth">Doreen Muth</name></noRegion><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M" last="Corman">Victor M. Corman</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Lattwein, Erik" sort="Lattwein, Erik" uniqKey="Lattwein E" first="Erik" last="Lattwein">Erik Lattwein</name><name sortKey="Memish, Ziad A" sort="Memish, Ziad A" uniqKey="Memish Z" first="Ziad A" last="Memish">Ziad A. Memish</name><name sortKey="Meyer, Benjamin" sort="Meyer, Benjamin" uniqKey="Meyer B" first="Benjamin" last="Meyer">Benjamin Meyer</name><name sortKey="Muller, Marcel A" sort="Muller, Marcel A" uniqKey="Muller M" first="Marcel A" last="Müller">Marcel A. Müller</name><name sortKey="Steinhagen, Katja" sort="Steinhagen, Katja" uniqKey="Steinhagen K" first="Katja" last="Steinhagen">Katja Steinhagen</name></country><country name="États-Unis"><region name="Indiana"><name sortKey="Al Tawfiq, Jaffar A" sort="Al Tawfiq, Jaffar A" uniqKey="Al Tawfiq J" first="Jaffar A" last="Al-Tawfiq">Jaffar A. Al-Tawfiq</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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